2Y8P
Crystal Structure of an Outer Membrane-Anchored Endolytic Peptidoglycan Lytic Transglycosylase (MltE) from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-25 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 123.324, 183.932, 35.292 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.118 - 1.995 |
| R-factor | 0.2055 |
| Rwork | 0.203 |
| R-free | 0.25180 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.048 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.120 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.060 | 0.300 |
| Number of reflections | 27762 | |
| <I/σ(I)> | 12.9 | 3.59 |
| Completeness [%] | 99.6 | 97.6 |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 28% POLYETHYLENE GLYCOL 4000, 0.1 M TRIS PH 8.4, 0.2 M MAGNESIUM CHLORIDE |
