2Y89
CRYSTAL STRUCTURE OF MYCOBACTERIUM TUBERCULOSIS PHOSPHORIBOSYL ISOMERASE A (VARIANT D11N)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-03 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 3 2 |
| Unit cell lengths | 141.534, 141.534, 141.534 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 100.000 - 2.500 |
| R-factor | 0.21315 |
| Rwork | 0.211 |
| R-free | 0.24990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.040 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.740 | 2.520 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.100 | 0.720 |
| Number of reflections | 17377 | |
| <I/σ(I)> | 23.86 | 3.12 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.14 | 14.44 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M BIS-TRIS PH 7.5, 2.0M AMMONIUM SULFATE. |
