2Y84

DntR Inducer Binding Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	81.200, 111.500, 213.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.229 - 2.800
R-factor	0.2191
Rwork	0.218
R-free	0.24440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1utb
RMSD bond length	0.003
RMSD bond angle	0.750
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.500	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.150	0.500
Number of reflections	47128
<I/σ(I)>	10.2	2.3
Completeness [%]	97.5	93.6
Redundancy	4.5	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				0.2 M NA/K TARTRATE, 0.1 M TRIS PH 8.0, 33% PEG 8000.