2Y7Y

APLYSIA CALIFORNICA ACHBP IN APO STATE

Replaces:  2W8E

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-10-01
Detector	ADSC CCD
Spacegroup name	C 2 2 21
Unit cell lengths	135.524, 175.935, 128.914
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.993 - 1.895
R-factor	0.1891
Rwork	0.187
R-free	0.22740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2c9t
RMSD bond length	0.009
RMSD bond angle	1.194
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.060	2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.090	0.600
Number of reflections	121101
<I/σ(I)>	5.5	1.1
Completeness [%]	99.8	100
Redundancy	3.4	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1