2Y7O

Structure of N-terminal domain of Candida albicans als9-2 - G299W mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-05-16
Detector	ADSC QUANTUM 315r
Spacegroup name	P 21 21 21
Unit cell lengths	63.370, 68.310, 89.350
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.510 - 1.750
R-factor	0.19685
Rwork	0.195
R-free	0.22304
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.027
RMSD bond angle	2.185
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.450	1.690
High resolution limit [Å]	1.590	1.590
Rmerge	0.070	0.450
Number of reflections	32766
<I/σ(I)>	20.7	4.6
Completeness [%]	96.8	89.4
Redundancy	10	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1