2Y7L
Structure of N-terminal domain of Candida albicans Als9-2 in complex with human fibrinogen gamma peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-25 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.581, 69.273, 122.223 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.640 - 1.490 |
| R-factor | 0.19771 |
| Rwork | 0.196 |
| R-free | 0.22429 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2y7m |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.096 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.520 |
| High resolution limit [Å] | 1.490 | 1.490 |
| Rmerge | 0.130 | 0.410 |
| Number of reflections | 50160 | |
| <I/σ(I)> | 36.4 | 9.3 |
| Completeness [%] | 98.0 | 86.6 |
| Redundancy | 13.7 | 9.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 0.2MM AMMONIUM FLUORIDE, 20% PEG3350, PH 6.2 |
