2Y7D
Crystal structure of the 3-keto-5-aminohexanoate cleavage enzyme (Kce) from Candidatus Cloacamonas acidaminovorans (orthorombic form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2008-03-27 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.870, 100.820, 147.430 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.450 - 1.590 |
| R-factor | 0.1585 |
| Rwork | 0.158 |
| R-free | 0.17400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PARTIAL MODEL FROM SEMET MAD PHASING IN DIFFERENT SPACE GROUP |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.000 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | BUSTER (2.9.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.470 | 1.680 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Rmerge | 0.070 | 0.500 |
| Number of reflections | 147835 | |
| <I/σ(I)> | 16.5 | 3 |
| Completeness [%] | 99.3 | 95.7 |
| Redundancy | 6.7 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 30% PEG8000, 0.08 M NA ACETATE, 100 MM NA-HEPES PH 7.5 . |
