2Y7B
Crystal structure of the PH domain of human Actin-binding protein anillin ANLN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-30 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.300, 59.417, 64.717 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.450 - 1.900 |
| R-factor | 0.19518 |
| Rwork | 0.192 |
| R-free | 0.26211 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1FHW 1u27 1u29 1u2b 2r0d |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.552 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.940 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.140 | 0.960 |
| Number of reflections | 10888 | |
| <I/σ(I)> | 9.1 | 2 |
| Completeness [%] | 99.6 | 99.8 |
| Redundancy | 4.7 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20% PEG3350, 100 MM BIS-TRIS PH 5.5, 40% ACETONITRILE |
