2Y79
STRUCTURE OF THE FIRST GAF DOMAIN E87A MUTANT OF MYCOBACTERIUM TUBERCULOSIS DOSS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6C1 |
| Synchrotron site | PAL/PLS |
| Beamline | 6C1 |
| Temperature [K] | 294 |
| Detector technology | CCD |
| Collection date | 2008-12-02 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 35.932, 86.913, 100.973 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.390 - 1.800 |
| R-factor | 0.19913 |
| Rwork | 0.197 |
| R-free | 0.24808 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2w3d |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.491 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 31.400 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.070 | 0.320 |
| Number of reflections | 29661 | |
| <I/σ(I)> | 32.3 | 4.3 |
| Completeness [%] | 98.2 | 86.9 |
| Redundancy | 6.8 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 19% PEG 6000, 200 MM CALCIUM ACETATE, 100 MM TRIS PH 7.0. |
