2Y74
THE CRYSTAL STRUCTURE OF HUMAN SOLUBLE PRIMARY AMINE OXIDASE AOC3 IN THE OFF-COPPER CONFORMATION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 183 |
| Detector technology | CCD |
| Collection date | 2006-04-21 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 225.800, 225.800, 218.700 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.207 - 2.950 |
| R-factor | 0.1805 |
| Rwork | 0.179 |
| R-free | 0.21380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1us1 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.211 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.500 | 3.050 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.140 | 0.480 |
| Number of reflections | 69215 | |
| <I/σ(I)> | 13.9 | 4.3 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 7.3 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 0.7M POTASSIUM/SODIUM TARTRATE, 100MM IMIDAZOLE (PH 7.4), 200MM NACL |
