2Y72
Crystal structure of the PKD Domain of Collagenase G from Clostridium Histolyticum at 1.18 Angstrom Resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-12 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 68.205, 59.300, 55.280 |
| Unit cell angles | 90.00, 125.61, 90.00 |
Refinement procedure
| Resolution | 44.940 - 1.180 |
| R-factor | 0.14734 |
| Rwork | 0.146 |
| R-free | 0.17997 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2c26 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.805 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.510 | 1.240 |
| High resolution limit [Å] | 1.180 | 1.180 |
| Rmerge | 0.070 | 0.300 |
| Number of reflections | 56646 | |
| <I/σ(I)> | 13.9 | 3.2 |
| Completeness [%] | 96.4 | 78.4 |
| Redundancy | 6 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 2.5M C3H2O4NA2, 10MM CACL2, PH 7.5 |
