2Y6V

Peroxisomal alpha-beta-hydrolase Lpx1 (Yor084w) from Saccharomyces cerevisiae (crystal form I)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2008-09-03
Detector	RIGAKU CCD
Spacegroup name	C 1 2 1
Unit cell lengths	140.480, 87.770, 125.040
Unit cell angles	90.00, 95.10, 90.00

Refinement procedure

Resolution	19.750 - 2.830
R-factor	0.20938
Rwork	0.207
R-free	0.25408
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	ENSEMBLE OF TEN ALPHA-BETA HYDROLASES AS POLY-ALA MODELS TRIMMED TO THE COMMON CORE.
RMSD bond length	0.016
RMSD bond angle	1.475
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.900
High resolution limit [Å]	2.830	2.830
Rmerge	0.090	0.400
Number of reflections	34876
<I/σ(I)>	15	2.97
Completeness [%]	95.6	68.4
Redundancy	3.6	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	HANGING OR SITTING DROP VAPOR DIFFUSION AT 20 DEGREES CELSIUS. 4 UL OF PROTEIN (7.9 MG/ML) MIXED WITH 2UL RESERVOIR (0.1 M HEPES, PH 7.5, 10 % PEG 8000).