2Y6M

Crystal structure of EphA4 kinase domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2010-04-28
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 21 21 21
Unit cell lengths	32.513, 91.045, 97.738
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.851 - 1.700
R-factor	0.1764
Rwork	0.175
R-free	0.20320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2hel
RMSD bond length	0.007
RMSD bond angle	0.993
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AMoRE
Refinement software	PHENIX ((PHENIX.REFINE: 1.7.2_869))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.850	1.790
High resolution limit [Å]	1.700	1.700
Rmerge	0.046	0.250
Number of reflections	31531
<I/σ(I)>	13.1	4.1
Completeness [%]	97.2	97.3
Redundancy	2.8	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				AMMONIUM SULFATE, BIS TRIS PH5.5, PEG 10 K