2Y6G

Cellopentaose binding mutated (X-2 L110F) CBM4-2 Carbohydrate Binding Module from a Thermostable Rhodothermus marinus Xylanase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-2
Synchrotron site	MAX II
Beamline	I911-2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-05-05
Detector	MARRESEARCH SX-165
Spacegroup name	P 21 21 21
Unit cell lengths	48.560, 49.720, 62.520
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.300
R-factor	0.132
R-free	0.18300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2y6h
RMSD bond length	0.011
RMSD bond angle	0.030
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	REFMAC
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.330
High resolution limit [Å]	1.300	1.300
Rmerge	0.040	0.470
Number of reflections	36185
<I/σ(I)>	19.7	3.2
Completeness [%]	95.4	91.9
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6		0.1 M MIB BUFFER PH 6.0, 20 % PEG 1500