2Y6B
Ascorbate Peroxidase R38K mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-11-30 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 82.707, 82.707, 75.026 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.200 - 1.900 |
| R-factor | 0.17838 |
| Rwork | 0.175 |
| R-free | 0.24074 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oag |
| RMSD bond length | 0.016 |
| RMSD bond angle | 6.200 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.200 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.040 | 0.220 |
| Number of reflections | 20947 | |
| <I/σ(I)> | 17.8 | 3.6 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
