2Y6A
Ascorbate Peroxidase R38A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-09-19 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 82.707, 82.707, 75.026 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.130 - 2.000 |
| R-factor | 0.13437 |
| Rwork | 0.130 |
| R-free | 0.20846 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oag |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.660 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.060 | 0.290 |
| Number of reflections | 21100 | |
| <I/σ(I)> | 26.5 | 4.8 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 3.8 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
