2Y64

Xylopentaose binding mutated (X-2 L110F) CBM4-2 Carbohydrate Binding Module from a Thermostable Rhodothermus marinus Xylanase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-2
Synchrotron site	MAX II
Beamline	I911-2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-03-10
Detector	MARRESEARCH SX-165
Spacegroup name	P 21 21 21
Unit cell lengths	48.460, 50.680, 62.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.400
R-factor	0.143
R-free	0.20000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2y6h
RMSD bond length	0.004
RMSD bond angle	0.020
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.440
High resolution limit [Å]	1.400	1.400
Rmerge	0.050	0.640
Number of reflections	30301
<I/σ(I)>	20.6	3.7
Completeness [%]	97.7	96.2
Redundancy	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6		0.1 M MIB BUFFER PH 6.0, 25 % W/V PEG 1500.