2Y5W

Crystal structure of Drosophila melanogaster kinesin-1 motor domain dimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	93
Detector technology	CCD
Collection date	2009-12-11
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	P 21 21 21
Unit cell lengths	76.890, 89.180, 138.350
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.740 - 2.700
R-factor	0.21384
Rwork	0.210
R-free	0.28883
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bg2
RMSD bond length	0.012
RMSD bond angle	1.829
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.840
High resolution limit [Å]	2.700	2.700
Rmerge	0.080	0.400
Number of reflections	35890
<I/σ(I)>	12	3.6
Completeness [%]	99.9	100
Redundancy	4.1	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.8		24 % POLYETHYLENE GLYCOL-3350, 0.15 M AMMONIUM SULFATE, AND 0.1 M HEPES SODIUM PH 7.8