2Y5N
Structure of the mixed-function P450 MycG in complex with mycinamicin V in P21 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2009-07-15 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.752, 57.428, 101.929 |
| Unit cell angles | 90.00, 113.47, 90.00 |
Refinement procedure
| Resolution | 75.910 - 1.620 |
| R-factor | 0.1183 |
| Rwork | 0.115 |
| R-free | 0.18821 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2Y4H |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.904 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 93.500 | 1.700 |
| High resolution limit [Å] | 1.610 | 1.610 |
| Rmerge | 0.040 | 0.300 |
| Number of reflections | 79430 | |
| <I/σ(I)> | 16.2 | 2.2 |
| Completeness [%] | 70.4 | 11.1 |
| Redundancy | 3.5 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 296 | 7% PEG 4000, 0.025 M MG ACETATE, 23 DEGREES C, pH 7.0 |
