2Y5I
S100Z from zebrafish in complex with calcium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2004-04-17 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 75.611, 132.250, 58.209 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 66.130 - 2.030 |
| R-factor | 0.1737 |
| Rwork | 0.171 |
| R-free | 0.22677 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cga |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.583 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0086) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.100 | |
| High resolution limit [Å] | 2.030 | 2.030 |
| Rmerge | 0.100 | 0.340 |
| Number of reflections | 38644 | |
| <I/σ(I)> | 24.3 | 5.8 |
| Completeness [%] | 98.8 | 85.1 |
| Redundancy | 10.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 0.1M SODIUM ACETATE; 10% PEG 1.5K; 0.2M CACL2, 20% ISOPROPANOL., pH 5.0 |
