2Y4V
CRYSTAL STRUCTURE OF HUMAN CALMODULIN IN COMPLEX WITH A DAP KINASE-1 MUTANT (W305Y) PEPTIDE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-01 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 65.561, 33.923, 74.257 |
| Unit cell angles | 90.00, 92.21, 90.00 |
Refinement procedure
| Resolution | 74.200 - 1.800 |
| R-factor | 0.22954 |
| Rwork | 0.229 |
| R-free | 0.23825 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yr5 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.712 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.590 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.050 | 0.220 |
| Number of reflections | 14844 | |
| <I/σ(I)> | 8.9 | 3.3 |
| Completeness [%] | 96.9 | 96.8 |
| Redundancy | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1 M HEPES PH 7.5, 25(W/V) PEG 3000 |
