2Y4U
Crystal structure of human P58(IPK) in space group P312
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 3 1 2 |
| Unit cell lengths | 124.620, 124.620, 92.220 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.930 - 3.200 |
| R-factor | 0.24626 |
| Rwork | 0.244 |
| R-free | 0.28631 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ieg |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.259 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.370 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.090 | 0.600 |
| Number of reflections | 12967 | |
| <I/σ(I)> | 8.9 | 2.1 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.6 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M TRIS, PH 8.5, 1.3 M SUCCINIC ACID, 1% (W/V) PEG MME 2000. |
