2Y4P
Dimeric structure of DAPK-1 catalytic domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-15 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.940, 73.700, 120.280 |
| Unit cell angles | 90.00, 101.24, 90.00 |
Refinement procedure
| Resolution | 117.970 - 2.650 |
| R-factor | 0.25465 |
| Rwork | 0.252 |
| R-free | 0.31242 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2w4j |
| RMSD bond length | 0.036 |
| RMSD bond angle | 1.787 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.510 | 2.790 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.110 | 0.500 |
| Number of reflections | 41340 | |
| <I/σ(I)> | 10.1 | 3.1 |
| Completeness [%] | 98.3 | 97.8 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 0.1 M TRIS PH 8.0, 0.1 M MGCL2, 15% (W/V) PEG 4000, 0.5 MM AMPPCP, 0.5 MM PMSF. |
