2Y3X
Catalytic domain of mouse 2',3'-cyclic nucleotide 3'- phosphodiesterase, complexed with sulfate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-14 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 31 |
| Unit cell lengths | 82.430, 82.430, 86.310 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 71.400 - 2.100 |
| R-factor | 0.19162 |
| Rwork | 0.190 |
| R-free | 0.22750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xmi |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.554 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.040 | 0.560 |
| Number of reflections | 38236 | |
| <I/σ(I)> | 19.98 | 2.5 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 3.85 | 3.83 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 3.5 | 50MM NA-CITRATE PH 3.5, 2.9M AMMONIUM SULFATE, 10% PEG 550 MME |
