2Y3S
Structure of the tirandamycine-bound FAD-dependent tirandamycin oxidase TamL in C2 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2010-12-04 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 162.046, 105.786, 70.971 |
| Unit cell angles | 90.00, 110.67, 90.00 |
Refinement procedure
| Resolution | 86.760 - 1.670 |
| R-factor | 0.16358 |
| Rwork | 0.161 |
| R-free | 0.22039 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2y08 |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.096 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.760 | 1.760 |
| High resolution limit [Å] | 1.670 | 1.670 |
| Rmerge | 0.110 | 0.510 |
| Number of reflections | 120184 | |
| <I/σ(I)> | 6.2 | 1.4 |
| Completeness [%] | 92.9 | 64.2 |
| Redundancy | 3.8 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 296 | 9% PEG 4000, 0.2M MGSO4, 23 DEGREES C., pH 7.5 |
