2Y3N

Type II cohesin-dockerin domain from Bacteroides cellolosolvens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	287
Detector technology	CCD
Detector	ADSC QUANTUM 315r
Spacegroup name	P 1 21 1
Unit cell lengths	43.410, 116.120, 45.160
Unit cell angles	90.00, 112.45, 90.00

Refinement procedure

Resolution	41.740 - 1.900
R-factor	0.16641
Rwork	0.163
R-free	0.22491
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1tyj
RMSD bond length	0.022
RMSD bond angle	1.696
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.5.0110)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.740	41.740
High resolution limit [Å]	1.900	1.900
Rmerge	0.090	0.330
Number of reflections	27197
<I/σ(I)>	6.5	3.2
Completeness [%]	83.7	83.7
Redundancy	2.4	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1