2Y3J
Structure of segment AIIGLM from the amyloid-beta peptide (Ab, residues 30-35)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 9.560, 15.640, 45.230 |
| Unit cell angles | 89.87, 90.02, 89.91 |
Refinement procedure
| Resolution | 45.230 - 1.990 |
| R-factor | 0.22462 |
| Rwork | 0.222 |
| R-free | 0.26710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.710 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0081) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.230 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.100 | 0.190 |
| Number of reflections | 1679 | |
| <I/σ(I)> | 4.91 | 2.11 |
| Completeness [%] | 97.5 | 88.5 |
| Redundancy | 1.73 | 1.63 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | AB3035 WAS DISSOLVED IN WATER AT 1MG/ML AND MIXED WITH 2 M SODIUM CHLORIDE, pH 7 |
