2Y3F

Traptavidin, biotin bound form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	CCD
Collection date	2009-12-06
Detector	ADSC CCD
Spacegroup name	P 42 21 2
Unit cell lengths	57.340, 57.340, 77.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.120 - 1.493
R-factor	0.1358
Rwork	0.135
R-free	0.15150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2y3e
RMSD bond length	0.009
RMSD bond angle	1.482
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.780	1.570
High resolution limit [Å]	1.490	1.490
Rmerge	0.070	0.080
Number of reflections	21720
<I/σ(I)>	26	19.6
Completeness [%]	100.0	99.9
Redundancy	7	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5		27% PEG 4000, 0.25M MGCL2, 0.1M TRIS-HCL PH 8.5