2Y3E
Traptavidin, apo-form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-06 |
| Detector | ADSC CCD |
| Spacegroup name | I 41 |
| Unit cell lengths | 57.594, 57.594, 183.354 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.474 - 1.449 |
| R-factor | 0.1353 |
| Rwork | 0.134 |
| R-free | 0.17820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1swb |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.934 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.470 | 1.530 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.050 | 0.620 |
| Number of reflections | 51553 | |
| <I/σ(I)> | 13.6 | 2 |
| Completeness [%] | 97.8 | 87.1 |
| Redundancy | 3.6 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 12% PEG 8000, 9% ETHYLENE GLYCOL, 0.1 M HEPES PH 7.5 |
