2Y3D
Zn-bound form of Cupriavidus metallidurans CH34 CnrXs
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-15 |
| Detector | MARRESEARCH |
| Spacegroup name | I 4 3 2 |
| Unit cell lengths | 155.721, 155.721, 155.721 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.953 - 2.300 |
| R-factor | 0.2096 |
| Rwork | 0.207 |
| R-free | 0.25930 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.169 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.950 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.090 | 0.400 |
| Number of reflections | 14718 | |
| <I/σ(I)> | 5.4 | 2 |
| Completeness [%] | 99.8 | 99 |
| Redundancy | 11.7 | 11.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1M HEPES PH7.5 10% PEG 6000 1M LICL |
