2Y39
Ni-bound form of Cupriavidus metallidurans CH34 CnrXs
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-10 |
| Detector | ADSC CCD |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 32.198, 32.198, 195.986 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.589 - 1.410 |
| R-factor | 0.1804 |
| Rwork | 0.179 |
| R-free | 0.19780 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.064 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.200 | 1.480 |
| High resolution limit [Å] | 1.410 | 1.410 |
| Rmerge | 0.040 | 0.150 |
| Number of reflections | 21101 | |
| <I/σ(I)> | 13.2 | 5.2 |
| Completeness [%] | 95.6 | 92.1 |
| Redundancy | 13.8 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0,1M HEPES PH7,5 0.6 M SODIUM PHOSPHATE, 1M POTASSIUM PHOSPHATE, pH 7.5 |
