2Y38

LAMININ ALPHA5 CHAIN N-TERMINAL FRAGMENT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 62
Unit cell lengths	116.400, 116.400, 112.300
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.000 - 2.900
R-factor	0.2538
Rwork	0.254
R-free	0.29250
Structure solution method	MIRAS
Starting model (for MR)	NONE
RMSD bond length	0.008
RMSD bond angle	1.800
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	autoSHARP
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	3.060
High resolution limit [Å]	2.900	2.900
Rmerge	0.140	0.680
Number of reflections	19173
<I/σ(I)>	10.7	3.1
Completeness [%]	100.0	100
Redundancy	11	11.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1