2Y2Y
Oxidised form of E. coli CsgC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-15 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 79.490, 36.430, 39.520 |
| Unit cell angles | 90.00, 117.45, 90.00 |
Refinement procedure
| Resolution | 21.620 - 2.000 |
| R-factor | 0.2647 |
| Rwork | 0.261 |
| R-free | 0.32842 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xsk |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.049 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.800 | 2.040 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.060 | 0.200 |
| Number of reflections | 6779 | |
| <I/σ(I)> | 14.4 | 4.6 |
| Completeness [%] | 95.9 | 84 |
| Redundancy | 3.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20% (W/V) PEG 3350, 0.2 M AMMONIUM NITRATE. |
