2Y2X
Crystal Structure of Pseudomonas Aeruginosa OpdK with Vanillate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-22 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 82.142, 96.840, 121.557 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.907 - 1.650 |
| R-factor | 0.18 |
| Rwork | 0.180 |
| R-free | 0.20060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qtk |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.059 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.060 | 0.590 |
| Number of reflections | 111761 | |
| <I/σ(I)> | 2 | |
| Completeness [%] | 99.8 | 89 |
| Redundancy | 7.7 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.05 M SODIUM CHLORIDE, 0.1 M SODIUM CITRATE, 26% PEG 400, PH 5.5. |
