2Y2T

E. coli CsgC in reduced form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2009-04-29
Detector	RIGAKU SATURN 944
Spacegroup name	C 2 2 21
Unit cell lengths	36.972, 80.549, 70.563
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.270 - 2.300
R-factor	0.2385
Rwork	0.237
R-free	0.27281
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.021
RMSD bond angle	2.103
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.450
High resolution limit [Å]	2.300	2.400
Rmerge	0.050	0.160
Number of reflections	4927
<I/σ(I)>	23.2	6.2
Completeness [%]	100.0	100
Redundancy	4.3	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				25% (W/V) PEG 4000, 30% (V/V) ETHYLENE GLYCOL.