2Y2E
crystal structure of AmpD grown at pH 5.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-25 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 32 |
| Unit cell lengths | 67.982, 67.982, 93.125 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.521 - 2.000 |
| R-factor | 0.2062 |
| Rwork | 0.201 |
| R-free | 0.27180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2y28 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.115 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.500 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.090 | 0.260 |
| Number of reflections | 32782 | |
| <I/σ(I)> | 12.9 | 4.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 0.1 M TRIS PH 5.5, 0.1 M LI2SO4, 28% PEG 3350 |
