2Y26

Transmission defective mutant of Grapevine Fanleaf virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-11-08
Detector	ADSC QUANTUM 315r
Spacegroup name	P 21 3
Unit cell lengths	408.000, 408.000, 408.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.784 - 2.700
R-factor	0.1934
Rwork	0.193
R-free	0.21030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	EM MAP
RMSD bond length	0.009
RMSD bond angle	1.188
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	AMoRE
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.000	2.770
High resolution limit [Å]	2.700	2.700
Rmerge	0.130	0.910
Number of reflections	604343
<I/σ(I)>	17.25	1.31
Completeness [%]	98.9	91.7
Redundancy	10.7	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	LARGE CRYSTALS OF GFLV-TD WERE GROWN IN 10 DAYS AT 293K BY VAPOR DIFFUSION (1 MICROLITER SITTING DROPS) WITH A VIRUS SOLUTION AT 4 MG/ML AND A RESERVOIR CONTAINING 7% (M/V) PEG 3350, 0.1 M HEPES NA PH 7.5, 0.2 M PROLINE.