2Y25

Crystal structure of the myomesin domains My11-My13

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315
Spacegroup name	P 42
Unit cell lengths	155.201, 155.201, 106.344
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.950 - 3.500
R-factor	0.2186
Rwork	0.217
R-free	0.26580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2r15
RMSD bond length	0.014
RMSD bond angle	1.567
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.580
High resolution limit [Å]	3.500	3.500
Rmerge	0.080	0.290
Number of reflections	29917
<I/σ(I)>	10.83	2.4
Completeness [%]	93.8	82
Redundancy	2.7	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6		MES PH 6, 220 MM LI2SO4, 13% PEG 8K.