2Y22

Human AlphaB-crystallin Domain (residues 67-157)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2008-06-25
Detector	ADSC CCD
Spacegroup name	P 21 21 2
Unit cell lengths	67.280, 78.340, 131.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.890 - 3.700
R-factor	0.2162
Rwork	0.213
R-free	0.27700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2y1y
RMSD bond length	0.008
RMSD bond angle	1.070
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	BUSTER (2.8.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.400	3.900
High resolution limit [Å]	3.700	3.700
Rmerge	0.240	0.660
Number of reflections	7861
<I/σ(I)>	8.7	4
Completeness [%]	100.0	100
Redundancy	12.8	13.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9		SITTING DROPS WITH 20 MG/ML PROTEIN IN 25 MM TRIS, PH 8.5, 200 MM NACL EQUILIBRATED AGAINST 110 MM BICINE, PH 9.0, 55% MPD