2Y1Y

Human alphaB crystallin ACD(residues 71-157)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	CCD
Collection date	2009-05-09
Detector	ADSC CCD
Spacegroup name	P 43 2 2
Unit cell lengths	36.030, 36.030, 148.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.012 - 2.000
R-factor	0.2051
Rwork	0.204
R-free	0.22110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wj7
RMSD bond length	0.003
RMSD bond angle	0.673
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.010	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.100	0.320
Number of reflections	7272
<I/σ(I)>	25.6	11.6
Completeness [%]	100.0	100
Redundancy	25.4	26.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9		SITTING DROPS, 20 MG/ML PROTEIN IN 25 MM TRIS, PH8.5, 200 MM NACL. EQUILIBRATED AGAINST MOTHER LIQUOR OF 110 MM BICINE, PH 9.0, 40% MPD