2Y1Q
Crystal Structure of ClpC N-terminal Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER X8 PROTEUM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-24 |
| Detector | BRUKER |
| Spacegroup name | P 65 |
| Unit cell lengths | 84.916, 84.916, 31.633 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.795 - 1.500 |
| R-factor | 0.1711 |
| Rwork | 0.170 |
| R-free | 0.19880 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.418 |
| Data reduction software | BRUKER (PROTEUM2) |
| Data scaling software | BRUKER (PROTEUM2) |
| Phasing software | HKL2Map |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.600 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.040 | 0.410 |
| Number of reflections | 19614 | |
| <I/σ(I)> | 21.08 | 2.06 |
| Completeness [%] | 92.6 | 82.3 |
| Redundancy | 5.29 | 2.14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 2.0M (NH4)2SO4, 100MM SODIUM ACETATE, PH4.6 |
