2Y0H

Crystal Structure of Pseudomonas Aeruginosa OpdL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X6A
Synchrotron site	NSLS
Beamline	X6A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-10-12
Detector	MARRESEARCH QUANTUM 210
Spacegroup name	C 2 2 21
Unit cell lengths	91.227, 115.032, 100.632
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.690 - 2.200
R-factor	0.214
Rwork	0.212
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qtk
RMSD bond length	0.007
RMSD bond angle	1.088
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE: 1.6.4_486))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.030
High resolution limit [Å]	2.000	2.000
Rmerge	0.080	0.790
Number of reflections	34444
<I/σ(I)>	10	2
Completeness [%]	95.0	97
Redundancy	5	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				28% PEG 400, 0.05M SODIUM CITRATE, PH 5.5 .