2Y0G
X-ray structure of Enhanced Green Fluorescent Protein (EGFP)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-05 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.047, 62.183, 70.018 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.470 - 1.500 |
| R-factor | 0.13831 |
| Rwork | 0.136 |
| R-free | 0.18849 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1q4a |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.068 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.100 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.080 | 0.510 |
| Number of reflections | 36173 | |
| <I/σ(I)> | 13.5 | 2.4 |
| Completeness [%] | 99.6 | 98.5 |
| Redundancy | 4.7 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.2 | 50 MM HEPES PH 8.2, 24% PEG 4000, 50 MM MGCL2, 10 MM BETA-MERCAPTOEPTHANOL |
