2Y0A

Structure of DAPK1 construct residues 1-304

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-09-15
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	P 21 21 21
Unit cell lengths	50.220, 78.880, 111.470
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.600
R-factor	0.20336
Rwork	0.200
R-free	0.25913
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ig1
RMSD bond length	0.019
RMSD bond angle	1.793
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.6.0085)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.700
High resolution limit [Å]	2.600	2.600
Rmerge	0.100	0.650
Number of reflections	14084
<I/σ(I)>	12.3	2.3
Completeness [%]	92.9	79.8
Redundancy	4	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6		5% PEG 6000, 0.1M MES, PH 6.0