2Y08

Structure of the substrate-free FAD-dependent tirandamycin oxidase TamL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	110
Detector technology	CCD
Collection date	2010-08-21
Detector	ADSC QUANTUM 315r
Spacegroup name	P 21 21 21
Unit cell lengths	64.378, 129.558, 134.585
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	134.590 - 1.700
R-factor	0.14404
Rwork	0.141
R-free	0.20500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2WDX
RMSD bond length	0.024
RMSD bond angle	1.882
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	134.580	1.790
High resolution limit [Å]	1.700	1.700
Rmerge	0.060	0.370
Number of reflections	115270
<I/σ(I)>	11.2	1.4
Completeness [%]	92.7	64.3
Redundancy	4	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5	296	15% PEG, 0.2 M MGCL2 AND 0.1 M TRIS-HCL, PH 7.5, TEMPERATURE 23 DEGREES C.