2Y07
CRYSTAL STRUCTURE ANALYSIS OF THE ANTI-(4-HYDROXY-3-NITROPHENYL) - ACETYL MURINE GERMLINE MONOCLONAL ANTIBODY BBE6.12H3 FAB FRAGMENT IN COMPLEX WITH A PHAGE DISPLAY DERIVED DODECAPEPTIDE PPYPAWHAPGNI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 288 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-08-30 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 54.359, 60.829, 111.724 |
| Unit cell angles | 90.00, 94.79, 90.00 |
Refinement procedure
| Resolution | 23.620 - 2.400 |
| R-factor | 0.2344 |
| Rwork | 0.234 |
| R-free | 0.25720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NATIVE FAB STRUCTURE |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.782 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.620 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.100 | 0.120 |
| Number of reflections | 14326 | |
| <I/σ(I)> | 5 | 3.6 |
| Completeness [%] | 95.5 | 93.6 |
| Redundancy | 6.2 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.1 | 50 MM TRIS HCL, 0.025% SODIUM AZIDE, PEG 3350, pH 7.1 |
