2XZV
The cyanobacterial PP2C-like phosphatase tPphA requires three metals in the catalytic center for efficient catalysis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARRESEARCH |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 38.646, 151.436, 82.603 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.258 - 1.600 |
R-factor | 0.2115 |
Rwork | 0.210 |
R-free | 0.24160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2j82 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.019 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.260 | 1.700 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.040 | 0.320 |
Number of reflections | 30657 | |
<I/σ(I)> | 13.64 | 3 |
Completeness [%] | 94.1 | 76.2 |
Redundancy | 1 | 1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 30% PEG3350 0.2M CACL2 0.1M HEPES PH 7.4 |