2XZV
The cyanobacterial PP2C-like phosphatase tPphA requires three metals in the catalytic center for efficient catalysis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 38.646, 151.436, 82.603 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.258 - 1.600 |
| R-factor | 0.2115 |
| Rwork | 0.210 |
| R-free | 0.24160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2j82 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.019 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.260 | 1.700 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.040 | 0.320 |
| Number of reflections | 30657 | |
| <I/σ(I)> | 13.64 | 3 |
| Completeness [%] | 94.1 | 76.2 |
| Redundancy | 1 | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 30% PEG3350 0.2M CACL2 0.1M HEPES PH 7.4 |
