2XZS
Death associated protein kinase 1 residues 1-312
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-04 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.300, 49.610, 130.500 |
| Unit cell angles | 90.00, 94.52, 90.00 |
Refinement procedure
| Resolution | 43.360 - 2.000 |
| R-factor | 0.18464 |
| Rwork | 0.183 |
| R-free | 0.22471 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ig1 |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.825 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.080 | 0.340 |
| Number of reflections | 46826 | |
| <I/σ(I)> | 11.5 | 3.5 |
| Completeness [%] | 95.6 | 89.9 |
| Redundancy | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.2 M MAGNESIUM ACETATE TETRAHYDRATE, 0.1 M SODIUM CACODYLATE, PH 6.5 AND 10% PEG 8000 |
