2XZR
Escherichia coli Immunoglobulin-binding protein EibD 391-438 FUSED TO GCN4 ADAPTORS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 36.670, 36.670, 228.580 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.100 - 2.800 |
| R-factor | 0.26509 |
| Rwork | 0.260 |
| R-free | 0.30775 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wpq |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.060 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.100 | 2.970 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.070 | 0.250 |
| Number of reflections | 4931 | |
| <I/σ(I)> | 9.77 | 4.09 |
| Completeness [%] | 98.8 | 99.1 |
| Redundancy | 2.81 | 2.92 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 20% (V/V) 2-PROPANOL, 200 MM SODIUM CITRATE, 100 MM HEPES PH 7.5. |
