2XZJ
THE ASPERGILLUS FUMIGATUS SIALIDASE IS A KDNASE: STRUCTURAL AND MECHANISTIC INSIGHTS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.800, 58.070, 94.400 |
| Unit cell angles | 90.00, 99.92, 90.00 |
Refinement procedure
| Resolution | 12.380 - 1.840 |
| R-factor | 0.20361 |
| Rwork | 0.201 |
| R-free | 0.26214 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xcy |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.325 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 12.400 | 1.940 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.090 | 0.150 |
| Number of reflections | 70073 | |
| <I/σ(I)> | 9.8 | 5.3 |
| Completeness [%] | 98.0 | 95 |
| Redundancy | 3.1 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
